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af488 conjugated rat anti mouse β il 3 antibody Af488 Conjugated Rat Anti Mouse β Il 3 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/recombinant+rat+rr+il+3/Mouse+IL-3R+beta+Alexa+Fluor%C2%AE+488-conjugated+Antibody/pmc09369378-177-93-100 Average 92 stars, based on 1 article reviews
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Amgen
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Image Search Results
Journal: Frontiers in Immunology
Article Title: Skin CD4 + Memory T Cells Play an Essential Role in Acquired Anti-Tick Immunity through Interleukin-3-Mediated Basophil Recruitment to Tick-Feeding Sites
doi: 10.3389/fimmu.2017.01348
Figure Lengend Snippet: Interleukin-3 (IL-3) is necessary and sufficient for basophil accumulation at the tick-feeding site. (A) The transcriptional expression of indicated genes at uninfested skin, first or second tick-feeding site on day 2 of infestation is shown (mean ± SEM, n = 3 each). (B,C) Wild-type, Il4 −/− , or Il3 −/− mice were infested twice with ticks, and the number of basophils (mean ± SEM, n = 4 each) at the second tick-feeding site in each mouse strain was examined on day 2 of infestation. (D) Rag2 −/− mice were treated daily with subcutaneous administration of recombinant IL-3 (100 ng/injection/day) in the right flank and control PBS in the left flank for 10 days from day −7 to day 2, and on day 0, mice were primarily infested with ticks at the administration sites of both flanks. The number of basophils (mean ± SEM, n = 4 each) at the first tick-feeding site of each side of flank was examined on day 2 of infestation. All the data shown are representative of three independent experiments. N.S., not significant; * p < 0.05; *** p < 0.001.
Article Snippet: Following reagents were purchased from
Techniques: Expressing, Recombinant, Injection
Journal: Frontiers in Immunology
Article Title: Skin CD4 + Memory T Cells Play an Essential Role in Acquired Anti-Tick Immunity through Interleukin-3-Mediated Basophil Recruitment to Tick-Feeding Sites
doi: 10.3389/fimmu.2017.01348
Figure Lengend Snippet: Interleukin-3 (IL-3) produced by CD4 + T cells is responsible for basophil accumulation at the second tick-feeding site. (A) Splenic CD4 + T cells isolated from wild-type (WT) or Il3 −/− mice were adoptively transferred into Rag2 −/− mice, and recipient mice were infested twice with ticks. The number of CD4 + T cells (left panel) and basophils (right panel) at the second tick-feeding site was examined on day 2 of infestation (mean ± SEM, n = 4 each). (B) WT mice were infested twice with ticks, and the number of IL-3 + CD4 + T cells at the first and second tick-feeding sites (left panel) and the frequency of IL-3 + T cells among CD4 + T cells at the first and second tick-feeding sites (right panel) were determined (mean ± SEM, n = 3 each). All the data shown are representative of three independent experiments. N.S., not significant; * p < 0.05.
Article Snippet: Following reagents were purchased from
Techniques: Produced, Isolation
Journal: Frontiers in Immunology
Article Title: Skin CD4 + Memory T Cells Play an Essential Role in Acquired Anti-Tick Immunity through Interleukin-3-Mediated Basophil Recruitment to Tick-Feeding Sites
doi: 10.3389/fimmu.2017.01348
Figure Lengend Snippet: Interleukin-3 (IL-3) + CD4 + T cells at the second tick-feeding site show memory phenotypes, including T RM and T EM cells. (A) The expression of CD44 (open histogram) on IL-3 + CD4 + T cells accumulating at the second tick-feeding site of wild-type mice is shown. A shaded histogram indicates staining with isotype-matched control antibody. (B,C) The number of IL-3 + CD4 + T cells showing the phenotype of T CM (CD3 + CD62L + CD44 + ), T EM (CD3 + CD62L − CD44 + CD69 − ), or T RM (CD44 + CD62L − CD69 + ) on day 2 (B) and on day 0 (C) of the second infestation is shown (mean ± SEM, n = 3 each). All the data shown are representative of three independent experiments. n.d., not detected.
Article Snippet: Following reagents were purchased from
Techniques: Expressing, Staining