recombinant rat rr il 3 Search Results


90
Bio-Techne corporation recombinant rat il-3 protein
Recombinant Rat Il 3 Protein, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems recombinant murine rm interleukin
Recombinant Murine Rm Interleukin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
R&D Systems recombinant rat il 1β protein
Recombinant Rat Il 1β Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems af488 conjugated rat anti mouse β il 3 antibody
Af488 Conjugated Rat Anti Mouse β Il 3 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation recombinant rat il-3 protein, cf
Recombinant Rat Il 3 Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems antibody against opg mab5491
Antibody Against Opg Mab5491, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems interleukin 3
Interleukin 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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NSJ Bioreagents il-4 antibody
Il 4 Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Revvity recombinant il
<t>Interleukin-3</t> <t>(IL-3)</t> is necessary and sufficient for basophil accumulation at the tick-feeding site. (A) The transcriptional expression of indicated genes at uninfested skin, first or second tick-feeding site on day 2 of infestation is shown (mean ± SEM, n = 3 each). (B,C) Wild-type, Il4 −/− , or Il3 −/− mice were infested twice with ticks, and the number of basophils (mean ± SEM, n = 4 each) at the second tick-feeding site in each mouse strain was examined on day 2 of infestation. (D) Rag2 −/− mice were treated daily with subcutaneous administration of recombinant IL-3 (100 ng/injection/day) in the right flank and control PBS in the left flank for 10 days from day −7 to day 2, and on day 0, mice were primarily infested with ticks at the administration sites of both flanks. The number of basophils (mean ± SEM, n = 4 each) at the first tick-feeding site of each side of flank was examined on day 2 of infestation. All the data shown are representative of three independent experiments. N.S., not significant; * p < 0.05; *** p < 0.001.
Recombinant Il, supplied by Revvity, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Amgen recombinant rat scf
<t>Interleukin-3</t> <t>(IL-3)</t> is necessary and sufficient for basophil accumulation at the tick-feeding site. (A) The transcriptional expression of indicated genes at uninfested skin, first or second tick-feeding site on day 2 of infestation is shown (mean ± SEM, n = 3 each). (B,C) Wild-type, Il4 −/− , or Il3 −/− mice were infested twice with ticks, and the number of basophils (mean ± SEM, n = 4 each) at the second tick-feeding site in each mouse strain was examined on day 2 of infestation. (D) Rag2 −/− mice were treated daily with subcutaneous administration of recombinant IL-3 (100 ng/injection/day) in the right flank and control PBS in the left flank for 10 days from day −7 to day 2, and on day 0, mice were primarily infested with ticks at the administration sites of both flanks. The number of basophils (mean ± SEM, n = 4 each) at the first tick-feeding site of each side of flank was examined on day 2 of infestation. All the data shown are representative of three independent experiments. N.S., not significant; * p < 0.05; *** p < 0.001.
Recombinant Rat Scf, supplied by Amgen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems clones 4806
<t>Interleukin-3</t> <t>(IL-3)</t> is necessary and sufficient for basophil accumulation at the tick-feeding site. (A) The transcriptional expression of indicated genes at uninfested skin, first or second tick-feeding site on day 2 of infestation is shown (mean ± SEM, n = 3 each). (B,C) Wild-type, Il4 −/− , or Il3 −/− mice were infested twice with ticks, and the number of basophils (mean ± SEM, n = 4 each) at the second tick-feeding site in each mouse strain was examined on day 2 of infestation. (D) Rag2 −/− mice were treated daily with subcutaneous administration of recombinant IL-3 (100 ng/injection/day) in the right flank and control PBS in the left flank for 10 days from day −7 to day 2, and on day 0, mice were primarily infested with ticks at the administration sites of both flanks. The number of basophils (mean ± SEM, n = 4 each) at the first tick-feeding site of each side of flank was examined on day 2 of infestation. All the data shown are representative of three independent experiments. N.S., not significant; * p < 0.05; *** p < 0.001.
Clones 4806, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Kirin Brewery Company recombinant rat stem cell factor (scf)
<t>Interleukin-3</t> <t>(IL-3)</t> is necessary and sufficient for basophil accumulation at the tick-feeding site. (A) The transcriptional expression of indicated genes at uninfested skin, first or second tick-feeding site on day 2 of infestation is shown (mean ± SEM, n = 3 each). (B,C) Wild-type, Il4 −/− , or Il3 −/− mice were infested twice with ticks, and the number of basophils (mean ± SEM, n = 4 each) at the second tick-feeding site in each mouse strain was examined on day 2 of infestation. (D) Rag2 −/− mice were treated daily with subcutaneous administration of recombinant IL-3 (100 ng/injection/day) in the right flank and control PBS in the left flank for 10 days from day −7 to day 2, and on day 0, mice were primarily infested with ticks at the administration sites of both flanks. The number of basophils (mean ± SEM, n = 4 each) at the first tick-feeding site of each side of flank was examined on day 2 of infestation. All the data shown are representative of three independent experiments. N.S., not significant; * p < 0.05; *** p < 0.001.
Recombinant Rat Stem Cell Factor (Scf), supplied by Kirin Brewery Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+rat+rr+il+3/human+recombinant+stem+cell+factor/pmc02212237-17-26-34
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Image Search Results


Interleukin-3 (IL-3) is necessary and sufficient for basophil accumulation at the tick-feeding site. (A) The transcriptional expression of indicated genes at uninfested skin, first or second tick-feeding site on day 2 of infestation is shown (mean ± SEM, n = 3 each). (B,C) Wild-type, Il4 −/− , or Il3 −/− mice were infested twice with ticks, and the number of basophils (mean ± SEM, n = 4 each) at the second tick-feeding site in each mouse strain was examined on day 2 of infestation. (D) Rag2 −/− mice were treated daily with subcutaneous administration of recombinant IL-3 (100 ng/injection/day) in the right flank and control PBS in the left flank for 10 days from day −7 to day 2, and on day 0, mice were primarily infested with ticks at the administration sites of both flanks. The number of basophils (mean ± SEM, n = 4 each) at the first tick-feeding site of each side of flank was examined on day 2 of infestation. All the data shown are representative of three independent experiments. N.S., not significant; * p < 0.05; *** p < 0.001.

Journal: Frontiers in Immunology

Article Title: Skin CD4 + Memory T Cells Play an Essential Role in Acquired Anti-Tick Immunity through Interleukin-3-Mediated Basophil Recruitment to Tick-Feeding Sites

doi: 10.3389/fimmu.2017.01348

Figure Lengend Snippet: Interleukin-3 (IL-3) is necessary and sufficient for basophil accumulation at the tick-feeding site. (A) The transcriptional expression of indicated genes at uninfested skin, first or second tick-feeding site on day 2 of infestation is shown (mean ± SEM, n = 3 each). (B,C) Wild-type, Il4 −/− , or Il3 −/− mice were infested twice with ticks, and the number of basophils (mean ± SEM, n = 4 each) at the second tick-feeding site in each mouse strain was examined on day 2 of infestation. (D) Rag2 −/− mice were treated daily with subcutaneous administration of recombinant IL-3 (100 ng/injection/day) in the right flank and control PBS in the left flank for 10 days from day −7 to day 2, and on day 0, mice were primarily infested with ticks at the administration sites of both flanks. The number of basophils (mean ± SEM, n = 4 each) at the first tick-feeding site of each side of flank was examined on day 2 of infestation. All the data shown are representative of three independent experiments. N.S., not significant; * p < 0.05; *** p < 0.001.

Article Snippet: Following reagents were purchased from Biolegend: allophycocyanin (APC)-conjugated anti-CD200R3 (Ba13), anti-CD44 (IM7), Rat IgG2b, and κ (RTK4530); phycoerythrin (PE)-conjugated anti-IL-3 (MP2-8F8), anti-CD63 (NVG-2), Rat IgG1, κ (RTK2071), Rat IgG2a, and κ (RTK2758); fluorescein isothiocyanate (FITC)-conjugated anti-CD49b (HMα2) and anti-CD62L (MEL-14); PE/Cy7-conjugated anti-CD69 (H1.2F3); APC/Cy7-conjugated anti-CD3ε (145-2C11); Pacific Blue™-conjugated anti-CD117 (2B8); brilliant violet 421™-conjugated anti-CD4 (RM4.5); and biotin-conjugated anti-CD4 (GK1.5), anti-CD8a (53-6.7), and recombinant IL-3.

Techniques: Expressing, Recombinant, Injection

Interleukin-3 (IL-3) produced by CD4 + T cells is responsible for basophil accumulation at the second tick-feeding site. (A) Splenic CD4 + T cells isolated from wild-type (WT) or Il3 −/− mice were adoptively transferred into Rag2 −/− mice, and recipient mice were infested twice with ticks. The number of CD4 + T cells (left panel) and basophils (right panel) at the second tick-feeding site was examined on day 2 of infestation (mean ± SEM, n = 4 each). (B) WT mice were infested twice with ticks, and the number of IL-3 + CD4 + T cells at the first and second tick-feeding sites (left panel) and the frequency of IL-3 + T cells among CD4 + T cells at the first and second tick-feeding sites (right panel) were determined (mean ± SEM, n = 3 each). All the data shown are representative of three independent experiments. N.S., not significant; * p < 0.05.

Journal: Frontiers in Immunology

Article Title: Skin CD4 + Memory T Cells Play an Essential Role in Acquired Anti-Tick Immunity through Interleukin-3-Mediated Basophil Recruitment to Tick-Feeding Sites

doi: 10.3389/fimmu.2017.01348

Figure Lengend Snippet: Interleukin-3 (IL-3) produced by CD4 + T cells is responsible for basophil accumulation at the second tick-feeding site. (A) Splenic CD4 + T cells isolated from wild-type (WT) or Il3 −/− mice were adoptively transferred into Rag2 −/− mice, and recipient mice were infested twice with ticks. The number of CD4 + T cells (left panel) and basophils (right panel) at the second tick-feeding site was examined on day 2 of infestation (mean ± SEM, n = 4 each). (B) WT mice were infested twice with ticks, and the number of IL-3 + CD4 + T cells at the first and second tick-feeding sites (left panel) and the frequency of IL-3 + T cells among CD4 + T cells at the first and second tick-feeding sites (right panel) were determined (mean ± SEM, n = 3 each). All the data shown are representative of three independent experiments. N.S., not significant; * p < 0.05.

Article Snippet: Following reagents were purchased from Biolegend: allophycocyanin (APC)-conjugated anti-CD200R3 (Ba13), anti-CD44 (IM7), Rat IgG2b, and κ (RTK4530); phycoerythrin (PE)-conjugated anti-IL-3 (MP2-8F8), anti-CD63 (NVG-2), Rat IgG1, κ (RTK2071), Rat IgG2a, and κ (RTK2758); fluorescein isothiocyanate (FITC)-conjugated anti-CD49b (HMα2) and anti-CD62L (MEL-14); PE/Cy7-conjugated anti-CD69 (H1.2F3); APC/Cy7-conjugated anti-CD3ε (145-2C11); Pacific Blue™-conjugated anti-CD117 (2B8); brilliant violet 421™-conjugated anti-CD4 (RM4.5); and biotin-conjugated anti-CD4 (GK1.5), anti-CD8a (53-6.7), and recombinant IL-3.

Techniques: Produced, Isolation

Interleukin-3 (IL-3) + CD4 + T cells at the second tick-feeding site show memory phenotypes, including T RM and T EM cells. (A) The expression of CD44 (open histogram) on IL-3 + CD4 + T cells accumulating at the second tick-feeding site of wild-type mice is shown. A shaded histogram indicates staining with isotype-matched control antibody. (B,C) The number of IL-3 + CD4 + T cells showing the phenotype of T CM (CD3 + CD62L + CD44 + ), T EM (CD3 + CD62L − CD44 + CD69 − ), or T RM (CD44 + CD62L − CD69 + ) on day 2 (B) and on day 0 (C) of the second infestation is shown (mean ± SEM, n = 3 each). All the data shown are representative of three independent experiments. n.d., not detected.

Journal: Frontiers in Immunology

Article Title: Skin CD4 + Memory T Cells Play an Essential Role in Acquired Anti-Tick Immunity through Interleukin-3-Mediated Basophil Recruitment to Tick-Feeding Sites

doi: 10.3389/fimmu.2017.01348

Figure Lengend Snippet: Interleukin-3 (IL-3) + CD4 + T cells at the second tick-feeding site show memory phenotypes, including T RM and T EM cells. (A) The expression of CD44 (open histogram) on IL-3 + CD4 + T cells accumulating at the second tick-feeding site of wild-type mice is shown. A shaded histogram indicates staining with isotype-matched control antibody. (B,C) The number of IL-3 + CD4 + T cells showing the phenotype of T CM (CD3 + CD62L + CD44 + ), T EM (CD3 + CD62L − CD44 + CD69 − ), or T RM (CD44 + CD62L − CD69 + ) on day 2 (B) and on day 0 (C) of the second infestation is shown (mean ± SEM, n = 3 each). All the data shown are representative of three independent experiments. n.d., not detected.

Article Snippet: Following reagents were purchased from Biolegend: allophycocyanin (APC)-conjugated anti-CD200R3 (Ba13), anti-CD44 (IM7), Rat IgG2b, and κ (RTK4530); phycoerythrin (PE)-conjugated anti-IL-3 (MP2-8F8), anti-CD63 (NVG-2), Rat IgG1, κ (RTK2071), Rat IgG2a, and κ (RTK2758); fluorescein isothiocyanate (FITC)-conjugated anti-CD49b (HMα2) and anti-CD62L (MEL-14); PE/Cy7-conjugated anti-CD69 (H1.2F3); APC/Cy7-conjugated anti-CD3ε (145-2C11); Pacific Blue™-conjugated anti-CD117 (2B8); brilliant violet 421™-conjugated anti-CD4 (RM4.5); and biotin-conjugated anti-CD4 (GK1.5), anti-CD8a (53-6.7), and recombinant IL-3.

Techniques: Expressing, Staining